crystal violet उदाहरण वाक्य
उदाहरण वाक्य
- Gram staining uses two dyes : Crystal violet and Fuchsin or Safranin ( the counterstain ) to differentiate between Gram-positive bacteria ( large Peptidoglycan layer on outer surface of cell ) and Gram-negative bacteria.
- "Like MG [ malachite green ], CV [ crystal violet ] is readily absorbed into fish tissue from water exposure and is reduced metabolically by fish to the leuco moiety, leucocrystal violet ( LCV ).
- My problem is thus : the current protocol requires the removal of the medium ( DMEM, 10 % FBS, 1 % P / S antibiotics ) before addition of the crystal violet / ethanol solution.
- Gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells making the cell wall more porous and incapable of retaining the crystal violet stain.
- The decolorization step is critical and must be timed correctly; the crystal violet stain is removed from both gram-positive and negative cells if the decolorizing agent is left on too long ( a matter of seconds ).
- In an 1898 experiment using crystal violet as a specific stain, Benda first became aware of the existence of hundreds of these tiny bodies in the cytoplasm of eukaryotic cells and assumed that they reinforced the cell structure.
- The leuco dyes used in direct thermal paper are usually triaryl methane phthalide dyes, such as Yamamoto Blue 4450, or fluoran dyes, such as Pergascript Black 2C . A third widely used leuco dye is Crystal Violet lactone.
- Gram-positive bacteria retain the crystal violet dye, and thus are stained violet, while the Gram-negative bacteria do not; after washing, a counterstain is added ( commonly safranin or fuchsine ) that will stain these Gram-negative bacteria a pink color.
- It is one of the four components of basic fuchsine . ( The others are rosaniline, new fuchsine and magenta II . ) It is structurally related to other triarylmethane dyes called methyl violets including crystal violet, which feature methyl groups on nitrogen.
- The thermochromic droplets are actually a mixture of several chemicals crystal violet lactone ( the color-changing dye itself ), benzotriazole ( a weak acid ), and a quaternary ammonium salt of a fatty acid ( myristylammonium oleate ) dissolved in 1-dodecanol as solvent.