denaturant उदाहरण वाक्य
उदाहरण वाक्य
- The "'denaturant binding model "'assumes that there are specific but independent sites on the protein molecule ( folded or unfolded ) to which the denaturant binds with an effective ( average ) binding constant " k ".
- The "'solvent exchange model "'( also called the weak binding model or selective solvation ) of Schellman invokes the idea of an equilibrium between the water molecules bound to independent sites on protein and the denaturant molecules in solution.
- Ions that have a strong'salting in'effect such as I " and SCN " are strong denaturants, because they salt in the peptide group, and thus interact much more strongly with the unfolded form of a protein than with its native form.
- Ions that have a strong " salting in " effect such as I " and SCN " are strong denaturants, because they salt in the peptide group, and thus interact much more strongly with the unfolded form of a protein than with its native form.
- The fitting variables A _ { N } and A _ { U } are sometimes allowed to vary linearly with the solution conditions, e . g ., temperature or denaturant concentration, when the asymptotes of A are observed to vary linearly under strongly folding or strongly unfolding conditions.
- In fitting to a two-state model, the logarithm of the folding and unfolding rates is assumed to depend linearly on the denaturant concentration, thus resulting in the slopes m f and m u, called the folding and unfolding m-values, respectively ( also called the kinetic m-values ).
- A number of models have been proposed to explain this observation prominent among them being the "'denaturant binding model "', "'solvent-exchange model "'( both by John Schellman ) and the "'Linear Extrapolation Model "'( LEM; by Nick Pace ).
- Acetic acid is a denaturant that is sometimes used in combination with the other precipitating fixatives, such as Davidson's AFA . The alcohols, by themselves, are known to cause considerable shrinkage and hardening of tissue during fixation while acetic acid alone is associated with tissue swelling; combining the two may result in better preservation of tissue morphology.
- This model tries to answer the question of whether the denaturant molecules actually bind to the protein or they " seem " to be bound just because denaturants occupy about 20-30 % of the total solution volume at high concentrations used in experiments, i . e . non-specific effects and hence the term weak binding.
- This model tries to answer the question of whether the denaturant molecules actually bind to the protein or they " seem " to be bound just because denaturants occupy about 20-30 % of the total solution volume at high concentrations used in experiments, i . e . non-specific effects and hence the term weak binding.